Ultra-High Performance Liquid Chromatography and Its Applications.
This is the first resource to fully cover the instrumentation, method development, and applications of Ultra-High Performance Liquid Chromatography (U-HPLC). It details both the benefits and limitations of this method in the pharmaceutical industry, clinical research, the food industry, and environm...
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Full text (MCPHS users only) |
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| Main Author: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Chicester :
Wiley,
2013
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| Subjects: | |
| Local Note: | ProQuest Ebook Central |
MARC
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| 100 | 1 | |a Xu, Q. Alan. | |
| 245 | 1 | 0 | |a Ultra-High Performance Liquid Chromatography and Its Applications. |
| 260 | |a Chicester : |b Wiley, |c 2013. | ||
| 300 | |a 1 online resource (306 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Ultra-High Performance Liquid Chromatography and Its Applications -- Contents -- Preface -- Contributors -- Chapter 1 UHPLC Method Development -- 1.1 INTRODUCTION -- 1.2 METHOD DEVELOPMENT -- 1.2.1 Gather Sample Information and Define Method Goals -- 1.2.2 Scout Columns and Mobile Phases -- 1.2.3 Analyze Scouting Results and Select Separation Conditions -- 1.2.4 Optimize the Method -- 1.2.5 Validate the Method -- 1.2.6 Phase-Appropriate Method Development -- 1.2.7 Very High Pressure and Frictional Heating in UHPLC -- 1.2.8 Relevance of Various Instrumentation to Method Development -- 1.2.9 Method Resolution and Speed Requirements -- 1.2.10 Example 1.1 -- 1.2.11 Example 1.2 -- 1.3 CONVERSION OF AN HPLC METHOD TO UHPLC -- 1.3.1 Select a UHPLC Column with the Same Chemistry -- 1.3.2 Measure Dwell Volumes of HPLC and UHPLC Systems -- 1.3.3 Calculate Injection Volume, Flow Rate, Holding Time, and Gradient Segment Time -- 1.3.4 Perform Verification Experiment -- 1.3.5 Validate the Method -- 1.3.6 Example 1.3 -- 1.3.7 Example 1.4 -- 1.4 CONVERSION OF A UHPLC METHOD TO HPLC -- 1.5 SUMMARY -- REFERENCES -- Chapter 2 Method Transfer Between HPLC and UHPLC Platforms -- 2.1 INTRODUCTION -- 2.2 TRANSFERRING HPLC METHODS TO UHPLC -- 2.2.1 Fundamental Physical Relationships -- 2.2.2 Types of UHPLC Applications -- 2.2.3 Additional Fundamental Considerations -- 2.2.4 Transfer of Isocratic Methods from HPLC to UHPLC -- 2.2.5 Cautions on Transferring Isocratic Methods from HPLC to UHPLC -- 2.2.6 Transfer of Gradient Methods from HPLC to UHPLC -- 2.2.7 Cautions on the Scaling of Gradient Methods from HPLC to UHPLC -- 2.2.8 Case Studies and Recommendations -- 2.2.9 Final Discussion of Practical Challenges -- 2.2.10 Conclusions on the Transfer of Methods from HPLC to UHPLC -- 2.3 TRANSFERRING UHPLC METHODS TO HPLC PLATFORMS -- 2.3.1 UHPLC to Traditional HPLC Columns. | |
| 505 | 8 | |a 2.3.2 UHPLC to Superficially Porous Particle HPLC Columns -- 2.3.3 UHPLC to HPLC Method Transfer -- 2.4 TRANSFERRING LC METHODS TO OTHER LABS -- 2.4.1 UHPLC Instrument Issues Encountered During Method Transfers -- 2.4.2 Back to LC Basics -- REFERENCES -- Chapter 3 Practical Aspects of Ultrahigh Performance Liquid Chromatography -- 3.1 INTRODUCTION -- 3.2 EFFECT OF EXTRA-COLUMN VOLUME ON PERFORMANCE OF SUB-2 lM PARTICLE-PACKED COLUMNS IN UHPLC -- 3.2.1 Theoretical Considerations -- 3.2.2 Extra-Column Volumes and Column Volumes -- 3.2.3 Effect of Extra-Column Volume on Performance -- 3.3 COLUMN PRESSURE AND FRICTIONAL HEATING -- 3.3.1 Column Pressure vs. Particle Size -- 3.3.2 Phenomenon of Frictional Heating -- 3.3.3 Effect of Frictional Heating on Retention Factor -- 3.3.4 Factors Leading to Frictional Heating -- 3.3.5 How to Resolve Thermal Gradient Issues -- 3.3.6 Effect of Pressure on Mobile Phase Characteristics -- 3.3.7 Effect of Pressure on Retention Factor with Minimal Frictional Heating -- 3.4 METHOD TRANSFER BETWEEN UHPLC AND HPLC AND OTHER TIPS -- 3.4.1 Column Dimension and Particle Size -- 3.4.2 Column Chemistry and Mobile Phase -- 3.4.3 Flow Rate and Gradient Profile -- 3.4.4 Injection Volume -- 3.4.5 Dwell Volume -- 3.4.6 UV Detection -- 3.4.7 Other Tips -- 3.5 CONCLUDING REMARKS -- REFERENCES -- Chapter 4 Coupling UHPLC with MS: The Needs, Challenges, and Applications -- 4.1 INTRODUCTION -- 4.2 TECHNICAL REQUIREMENTS FOR THE COUPLING OF UHPLC WITH MS -- 4.2.1 Mobile Phase Flow Rate Compatibility -- 4.2.2 Acquisition Rate and Data Quality -- 4.2.3 Band-Broadening in UHPLC-MS Conditions -- 4.3 UHPLC-MS FOR BIOANALYTICAL ASSAYS -- 4.3.1 Selectivity Issues and Matrix Effects -- 4.3.2 Time Delivery Constraints -- 4.4 DRUG METABOLISM STUDIES USING UHPLC-MS -- 4.4.1 High-Throughput Metabolite Assays. | |
| 505 | 8 | |a 4.4.2 High-Resolution Metabolite Identification -- 4.5 MULTI-ANALYTE SCREENING WITH UHPLC-MS -- 4.5.1 Sample Preparation Procedures -- 4.5.2 UHPLC Conditions -- 4.5.3 Triple Quadrupole vs. Time-of-Flight Analyzers -- 4.6 UHPLC-MS IN METABOLOMICS -- 4.6.1 UHPLC-MS for Metabolomics in Human and Animal Tissues -- 4.6.2 UHPLC-MS for Metabolomics in Plant Extracts -- 4.7 ANALYSIS OF PROTEINS WITH UHPLC-MS -- 4.7.1 Issues and Solutions in Intact Protein Analysis -- 4.7.2 UHPLC-MS for Intact Protein Analysis -- 4.8 CONCLUSIONS -- REFERENCES -- Chapter 5 The Potential of Shell Particles in Fast Liquid Chromatography -- 5.1 INTRODUCTION -- 5.2 COLUMN EFFICIENCY -- 5.3 FAST LIQUID CHROMATOGRAPHY -- 5.3.1 Trends in Fast Liquid Chromatography -- 5.3.2 Adjusting Conventional Methods to Fast Separations (Geometrical Method Transfer -- 5.4 THE IMPACT OF EXTRA-COLUMN BAND-BROADENING IN FAST LIQUID CHROMATOGRAPHY -- 5.5 SHELL PARTICLES, THE INFLUENCE OF SHELL THICKNESS -- 5.6 THE EFFICIENCY OF COLUMNS PACKED WITH SHELL PARTICLES -- 5.6.1 The Mass Transfer Kinetics of Shell Particles -- 5.6.2 The Longitudinal Diffusion in Columns Packed with Shell Particles -- 5.6.3 The Eddy Dispersion in Columns Packed with Shell Particles -- 5.6.4 The Frictional Heating in Columns Packed with Shell Particles -- 5.6.5 Limited Efficiency When Shell Particles Packedin Narrow-Bore Columns -- 5.6.6 The Success of the New Generation Shell Particles -- 5.7 FAST SEPARATIONS BY APPLYING THE NEW GENERATION OF SHELL PARTICLES -- 5.8 APPLICATIONS OF COLUMNS PACKED WITH THE LATEST GENERATION OF SHELL PARTICLES -- 5.8.1 Pharmaceutical and Bioanalytical Applications -- 5.8.2 Food Analytical Applications -- 5.8.3 Environmental Analytical Applications -- 5.8.4 Multidimensional Separations -- 5.9 CONCLUSION -- REFERENCES -- Chapter 6 UHPLC Determination of Drugs of Abuse in Human Biological Matrices. | |
| 505 | 8 | |a 6.1 INTRODUCTION -- 6.2 CLASSES OF DRUGS AND ILLICIT SUBSTANCES -- 6.2.1 Amphetamines -- 6.2.2 Benzodiazepines -- 6.2.3 Cannabinoids -- 6.2.4 Cocaine Alkaloids -- 6.2.5 Designer Drugs -- 6.2.6 Ketamine -- 6.2.7 Lysergic Acid Diethylamide -- 6.2.8 Opiates and Opioids -- 6.2.9 Diuretics, b -Blockers, and Stimulants -- 6.3 DRUG METABOLIZATION IN THE HUMAN BODY -- 6.4 HUMAN MATRICES ANALYZED -- 6.4.1 Whole Blood, Plasma, and Serum -- 6.4.2 Fingerprints, Sebum, and Sweat -- 6.4.3 Hair -- 6.4.4 Meconium -- 6.4.5 Oral Fluid -- 6.4.6 Urine -- 6.5 PRETREATMENT AND ANALYSIS -- REFERENCES -- Chapter 7 UHPLC in the Analyses of Isoflavones and Flavonoids -- 7.1 INTRODUCTION -- 7.2 UHPLC IN POLYPHENOLIC COMPOUNDS DETERMINATION -- 7.2.1 Determination of Polyphenolic Compounds in Biological Fluids -- 7.2.2 Determination of Polyphenolic Compounds in Foods -- 7.2.3 Determination of Polyphenolic Compounds in Plant Extract -- 7.3 SUMMARY -- REFERENCES -- Chapter 8 UHPLC for Characterization of Protein Therapeutics -- 8.1 INTRODUCTION -- 8.2 PROTEIN CHARACTERIZATION AND LOT RELEASE TESTING -- 8.3 HPLC AND UHPLC -- 8.4 REVERSED-PHASE UHPLC FOR PROTEIN ANALYSIS -- 8.5 REVERSED-PHASE UHPLC FOR PEPTIDE MAP ANALYSIS -- 8.6 UHPLC FOR LC-MS AND LC-MS/MS APPLICATIONS -- 8.7 HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR GLYCAN PROFILING -- 8.8 HYDROPHOBIC INTERACTION CHROMATOGRAPHY FOR HYDROPHOBICITY ANALYSIS -- 8.9 ION EXCHANGE CHROMATOGRAPHY FOR CHARGE VARIANT ANALYSIS -- 8.10 SIZE EXCLUSION CHROMATOGRAPHY FOR SIZE HETEROGENEITY ANALYSIS -- 8.11 CONCLUSION -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 9 UHPLC/MS Analysis of Illicit Drugs -- 9.1 INTRODUCTION -- 9.2 APPLICATIONS OF UHPLC/MS IN ILLICIT DRUG ANALYSIS -- 9.2.1 Development of a UHPLC/MS Method for Simultaneous Identification of Multiple Drugs of Abuse. | |
| 505 | 8 | |a 9.2.2 Quantitative Analysis of Pseudoephedrine Tablets by UHPLC/MS -- 9.2.3 Identification of Cannabinoids in Baked Goods by UHPLC/MS -- 9.2.4 Identification of Psychotropic Substances in Mushrooms and Chocolate by UHPLC/MS -- 9.2.5 Identification of Lysergic Acid Diethylamide in Candy by UHPLC/MS -- 9.3 CONCLUSIONS -- REFERENCES -- Chapter 10 Ultra-High Performance Liquid Chromatography -- Mass Spectrometry and Its Application -- 10.1 INTRODUCTION -- 10.2 INSTRUMENTATION OF UHPLC-MS -- 10.2.1 UHPLC System -- 10.2.2 Mass Spectrometer -- 10.3 UHPLC-MS APPLICATIONS -- 10.3.1 UHPLC-MS Applications in Analysis of Traditional Chinese Medicines -- 10.3.2 UHPLC-MS Applications in Metabonomics -- 10.3.3 UHPLC-MS Applications in Drug Metabolism -- 10.3.4 UHPLC-MS Applications in Pharmacokinetics -- 10.4 CONCLUSIONS -- REFERENCES -- Index. | |
| 520 | |a This is the first resource to fully cover the instrumentation, method development, and applications of Ultra-High Performance Liquid Chromatography (U-HPLC). It details both the benefits and limitations of this method in the pharmaceutical industry, clinical research, the food industry, and environmental services. It covers U-HPLC topics coupled with ultra-violet detector (UV) and mass spectrometer (MS), instrumentation frequently used in solving complex molecules. This is an essential reference for scientists who utilize chromatographic techniques, including in academia, as well as pharmaceut. | ||
| 546 | |a English. | ||
| 588 | 0 | |a Print version record. | |
| 590 | |a ProQuest Ebook Central |b Ebook Central Academic Complete | ||
| 650 | 0 | |a Liquid chromatography. | |
| 650 | 2 | |a Chromatography, Liquid | |
| 650 | 7 | |a liquid chromatography. |2 aat | |
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