Applications of modern mass spectrometry. Volume 1

Applications of modern mass spectrometry. Volume 1 /

The first volume of the series presents 5 reviews: - Applications of mass spectrometry for the determination of the microbial crude protein synthesis in ruminants - Qualitative and quantitative LC-MS analysis in food proteins and peptides - Chemometrics as a powerful and complementary tool for ma...

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Bibliographic Details
Online Access: Full text (MCPHS users only)
Other Authors: Rahman, Atta-ur-, 1942- (Editor), Choudhary, Muhammad Iqbal (Editor), Musharraf, Syed Ghulam (Editor)
Format: Electronic eBook
Language:English
Published: Singapore : Bentham Science Publishers 2020
Series:Applications of Modern Mass Spectrometry Ser.
Subjects:
Mass spectrometry.
Mass Spectrometry
mass spectrometry.
Local Note:ProQuest Ebook Central
Table of Contents:
  • Cover
  • Title
  • Copyright
  • End User License Agreement
  • Contents
  • Preface
  • List of Contributors
  • Applications of Mass Spectrometry for the Determination of Microbial Crude Protein Synthesis in Ruminants
  • Lizbeth E. Robles-Jimenez1, Ivan Mendez Martinez1, Joaquim Balcells2 and Manuel Gonzalez-Ronquillo1,*
  • INTRODUCTION
  • MICROBIAL CRUDE PROTEIN SYNTHESIS
  • DIETARY NITROGEN FRACTIONS AND THEIR POTENTIAL USE BY RUMINANTS
  • METHODS OF DIRECTLY AND INDIRECTLY ESTIMATING MICROBIAL PROTEIN SYNTHESIS
  • CHARACTERISTICS AND USES OF 15N
  • Common Uses of 15N
  • 15N-labelled Forage
  • Ammonium Sulfate & Ammonium Chloride
  • Omasal Sampling and Analysis
  • Ruminal and Duodenal Samples
  • Liquid-associated and Solid-associated Microbes in the Rumen
  • Endogenous Excretion of Purine Derivatives
  • Extraction of Purine Bases and Derivatives
  • Extraction of Allantoin or Allantoic Acid
  • Isotope Abundance
  • Rumen 15N Pool Sizes in Excess of Background Levels
  • CONCLUSION
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • Qualitative and Quantitative LC-MS Analysis in Food Proteins and Peptides
  • Yi-Shen Zhu1,*, Zhonghong Li2 and Lulu Zhao1
  • INTRODUCTION
  • QUALITATIVE MEHTODS OF LC-MS ON FOOD PROTEINS AND PEPTIDES
  • The Primary Structure and Relative Molecular Mass of Proteins and Peptides
  • Intramolecular Disulfide Bonds Analysis of Proteins
  • Structural Analysis of PTMs Proteins and Peptides
  • QUANTITATIVE METHODS OF LC-MS ON FOOD PROTEINS AND PEPTIDES
  • Labeled Quantitation Methods
  • Chemical Labeling Method
  • Sulfhydryl Labeling Method
  • Carboxyl Labeling Method
  • Metabolic Labeling Method
  • 15N/14N Labeling Technology
  • SILAC Technology
  • Enzymatic Labeling Method
  • Application of 18O Labeling Method in Food Protein Research
  • Introduction of Multiple Reaction Monitoring (MRM)
  • Application of MRM in Proteins and Peptides Research
  • Label-Free Quantitation Method
  • Application of Label-Free Quantitation Method in Food Protein Research
  • Comparison of Two Approaches of Label-Free Quantitation Method
  • Comparison Between Labeled Quantitation and Label-Free Quantitation Methods
  • OUTLOOK
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • Chemometrics as a Powerful and Complementary Tool for Mass Spectrometry Applications in Life Sciences
  • Yahya Izadmanesh1,* and Jahan B. Ghasemi1,*
  • INTRODUCTION
  • MASS SPECTROMETRY OMICS DATA
  • METABOLOMIC MASS DATA
  • Experimental Design
  • Data Preprocessing
  • Noise Filtering and Baseline Correction
  • Peak Detection and Deconvolution
  • Normalization
  • Metabolites Identification
  • Resolution Algorithms
  • Variable Selection
  • Variable Ranking
  • Variable Subset Selection
  • Modeling of the Data
  • Unsupervised Methods
  • Supervised Methods
  • Non-linear Methods

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